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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA14 Polyclonal antibody

CA14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13736-1-AP

Synonyms

CA XIV, CA14, Carbonate dehydratase XIV, Carbonic anhydrase 14, carbonic anhydrase XIV

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CA14 Polyclonal antibody (13736-1-AP)

    WB analysis using 13736-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CA14 Polyclonal antibody (13736-1-AP)

    WB analysis using 13736-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using CA14 Polyclonal antibody (13736-1-AP)

    WB analysis of mouse eye using 13736-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse eye tissue, mouse retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13736-1-AP targets CA14 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA14 fusion protein Ag4673 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XIV
Calculated molecular weight 337 aa, 38 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC034412
Gene symbol CA14
Gene ID (NCBI) 23632
RRIDAB_2065836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbonic anhydrase XIV (CAXIV, Car14) is highly expressed in the hepatocyte with predominance in the canalicular membrane and its active site in the extracellular milieu. The CA XIV isoform may also be of importance for regulation of neuronal excitability and transport processes in brain.

Protocols

Product Specific Protocols
WB protocol for CA14 antibody 13736-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Glia

Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome

Authors - Sophie B Siems
View Article