CA2 Polyclonal antibody
CA2 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16961-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells |
| Positive IP detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 3 publications below |
Product Information
16961-1-AP targets CA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CA2 fusion protein Ag10680 相同性解析による交差性が予測される生物種 |
| Full Name | carbonic anhydrase II |
| Calculated molecular weight | 260 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC011949 |
| Gene symbol | CA2 |
| Gene ID (NCBI) | 760 |
| RRID | AB_2065857 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CA2 antibody 16961-1-AP | Download protocol |
| IP protocol for CA2 antibody 16961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells. | ||
Sci Rep SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma. | ||
J Inflamm Res M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs | ||
Bone Evidence that pyrophosphate acts as an extracellular signalling molecule to exert direct functional effects in primary cultures of osteoblasts and osteoclasts | ||
Front Mol Neurosci Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model. | ||
J Cancer Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Adrianna (Verified Customer) (11-01-2022) | Very good antibody, 1:1000 dilution incubated ON at 4 deg C gave a strong signal.
|
FH Isabel (Verified Customer) (07-03-2020) | Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.
 |
