Caspase 3(human specific) Monoclonal antibody

Name: Caspase 3(human specific) Monoclonal antibody
Brand: Proteintech
SKU: 66470-1-Ig

Caspase 3(human specific) Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1E7F9

Review Product

Cat no : 66470-1-Ig

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Validation Data Gallery

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AllWBIHCIF

WB analysis of Jurkat using 66470-1-Ig
Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 66470-1-Ig
Untreated and staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 66470-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-1-Ig (CASP3(human specific) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 66470-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-1-Ig (CASP3(human specific) antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66470-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-1-Ig (CASP3(human specific) antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 66470-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66470-1-Ig (Caspase 3(human specific) antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Cat No. 66470-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 67 publications below
IHC	See 2 publications below
IF	See 5 publications below

Product Information

66470-1-Ig targets Caspase 3(human specific) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, pig, zebrafish, sheep, cow
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Caspase 3(human specific) fusion protein Ag25029 相同性解析による交差性が予測される生物種
Full Name	caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight	277 aa, 32 kDa
Observed molecular weight	32-35 kDa, 19 kDa, 17 kDa
GenBank accession number	BC016926
Gene symbol	Caspase 3
Gene ID (NCBI)	836
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Casp3 (caspase 3), also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. This antibody is specific for human caspase 3, it can recognize the 32 kDa pro-caspase 3 as well as 17 and 19 kDa cleaved-caspase 3.

Protocols

Product Specific Protocols
WB protocol for Caspase 3(human specific) antibody 66470-1-Ig	Download protocol
IHC protocol for Caspase 3(human specific) antibody 66470-1-Ig	Download protocol
IF protocol for Caspase 3(human specific) antibody 66470-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Authors - Fei Yi View Article
rat	WB	Redox Biol Restoration of L-OPA1 alleviates acute ischemic stroke injury in rats via inhibiting neuronal apoptosis and preserving mitochondrial function. Authors - Yongxing Lai View Article
human	WB	Environ Int Chemical conjugation of FITC to track silica nanoparticles in vivo and in vitro: An emerging method to assess the reproductive toxicity of industrial nanomaterials. Authors - Junxia Li View Article
human	WB	Cancer Lett UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer. Authors - Dongying Wang View Article
rat	WB,IHC	Oxid Med Cell Longev Gastrodin Promotes the Survival of Random-Pattern Skin Flaps via Autophagy Flux Stimulation. Authors - Hongyu Chen View Article
human	WB	Oxid Med Cell Longev Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK. Authors - Ning Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 3(human specific) Monoclonal antibody

Caspase 3(human specific) Monoclonal Antibody for WB, IF, IHC, ELISA

Validation Data Gallery

WB analysis of Jurkat using 66470-1-Ig

WB analysis of Jurkat using 66470-1-Ig

WB analysis of HEK-293 using 66470-1-Ig

IHC staining of human breast cancer using 66470-1-Ig

IHC staining of human breast cancer using 66470-1-Ig

IF Staining of HeLa using 66470-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis.

Restoration of L-OPA1 alleviates acute ischemic stroke injury in rats via inhibiting neuronal apoptosis and preserving mitochondrial function.

Chemical conjugation of FITC to track silica nanoparticles in vivo and in vitro: An emerging method to assess the reproductive toxicity of industrial nanomaterials.

UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.

Gastrodin Promotes the Survival of Random-Pattern Skin Flaps via Autophagy Flux Stimulation.

Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.