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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for IF-P, FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human and More (1)

Applications

IF-P, FC, ELISA

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65150-1-Ig

Synonyms

CD45, B220, L CA, GP180, EC:3.1.3.48

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    IHC staining of human colon cancer using 65150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    IHC staining of human tonsillitis using 65150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    IHC staining of human tonsillitis using 65150-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65150-1-Ig (CD45RO antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    IF Staining of human colon cancer using 65150-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CD45RO antibody (65150-1-Ig, Clone: UCHL1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface co-stained with Atlantic Blue Anti-Human CD45RA and 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD45RO (UCHL1) (65150-1-Ig)

    FC experiment of human PBMCs using 65150-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive IF-P detected inhuman colon cancer tissue
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ELISASee 1 publications below

Product Information

65150-1-Ig targets CD45RO in IF-P, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol CD45
Gene ID (NCBI) 5788
RRIDAB_2881476
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IF protocol for CD45RO antibody 65150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseELISA

Curr Issues Mol Biol

Significance of CD10 for Mucosal Immunomodulation by β-Casomorphin-7 in Exacerbation of Ulcerative Colitis

Authors - Yoshihiro Miyagawa
View Article