Anti-Human CD68 (Y1/82A)
CD68 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b, kappa
Reactivity
Human and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra)
Conjugate
Unconjugated
CloneNo.
Y1/82A
Cat no : 65187-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | THP-1 cells |
| Positive FC (Intra) detected in | human PBMCs |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| FC | See 1 publications below |
Product Information
65187-1-Ig targets CD68 in WB, IHC, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Lysosomal contents of lung macrophages 相同性解析による交差性が予測される生物種 |
| Full Name | CD68 molecule |
| Calculated molecular weight | 37 kDa |
| GenBank accession number | BC015557 |
| Gene symbol | CD68 |
| Gene ID (NCBI) | 968 |
| RRID | AB_2918458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD68 antibody 65187-1-Ig | Download protocol |
| FC protocol for CD68 antibody 65187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway | ||
Phytomedicine Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice | ||
Int Immunopharmacol Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis | ||
Hepatology Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis | ||
Exp Neurol Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model | ||
Int Immunopharmacol Inhibition of aquaporin-9 ameliorates severe acute pancreatitis and associated lung injury by NLRP3 and Nrf2/HO-1 pathways |
