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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD9 Recombinant antibody

CD9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5K14

Cat no : 82105-1-RR

Synonyms

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD9 Recombinant antibody (82105-1-RR)

    WB analysis using 82105-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human urine exosomes tissue using CD9 Recombinant antibody (82105-1-RR)

    WB analysis of human urine exosomes using 82105-1-RR

    human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human lung cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human endometrial cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human endometrial cancer using 82105-1-RR

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD9 Recombinant antibody (82105-1-RR)

    IF Staining of MCF-7 using 82105-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82105-1-RR

    Affinity and Kinetic Characterization of 82105-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

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Tested Applications

Positive WB detected inHeLa cells, human urine exosomes tissue, L02 cells, A431 cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82105-1-RR targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD9 fusion protein Ag14546 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight23-27 kDa
GenBank accession numberBC011988
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 82105-1-RRDownload protocol
IHC protocol for CD9 antibody 82105-1-RRDownload protocol
IF protocol for CD9 antibody 82105-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols