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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CEP110 Polyclonal antibody (25235-1-AP)

    WB analysis of Jurkat using 25235-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25235-1-AP (CEP110 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CEP110 Polyclonal antibody (25235-1-AP)

    IF Staining of MDCK using 25235-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 25235-1-AP (CEP110 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CEP110 Polyclonal antibody

CEP110 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25235-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, ELISA

Synonyms

Centriolin, 110 kDa centrosomal protein, Centrosomal protein 1, centrosomal protein 110kDa, Centrosomal protein of 110 kDa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25235-1-AP targets CEP110 in WB, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18907

Product name: Recombinant human CEP110 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1996-2325 aa of BC137284

Sequence: VLAAEERVRTLQEEERWCESLEKTLSQTKRQLSEREQQLVEKSGELLALQKEADSMRADFSLLRNQFLTERKKAEKQVASLKEALKIQRSQLEKNLLEQKQENSCIQKEMATIELVAQDNHERARRLMKELNQMQYEYTELKKQMANQKDLERRQMEISDAMRTLKSEVKDEIRTSLKNLNQFLPELPADLEAILERNENLEGELESLKENLPFTMNEGPFEEKLNFSQVHIMDEHWRGEALREKLRHREDRLKAQLRHCMSKQAEVLIKGKRQTEGTLHSLRRQVDALGELVTSTSADSASSPSLSQLESSLTEDSQLGQNQEKNASAR

相同性解析による交差性が予測される生物種
Full Name centrosomal protein 110kDa
Calculated molecular weight 2325 aa, 269 kDa
Observed molecular weight270 kDa
GenBank accession numberBC137284
Gene Symbol CEP110
Gene ID (NCBI) 11064
RRIDAB_2879979
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7A1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CEP110 antibody 25235-1-APDownload protocol
IF protocol for CEP110 antibody 25235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols