CHST3 Polyclonal antibody

CHST3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18242-1-AP

Synonyms

C6ST, C6ST 1, C6ST1, Chondroitin 6 sulfotransferase, CHST3, GST 0, HSD


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Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18242-1-AP targets CHST3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST3 fusion protein Ag13008 相同性解析による交差性が予測される生物種
Full Name carbohydrate (chondroitin 6) sulfotransferase 3
Calculated molecular weight 479 aa, 55 kDa
Observed molecular weight55-67 kDa
GenBank accession numberBC093690
Gene symbol CHST3
Gene ID (NCBI) 9469
RRIDAB_10694672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHST3 antibody 18242-1-APDownload protocol
IHC protocol for CHST3 antibody 18242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.

Authors - Laura Hobohm
humanIHC

Osteoarthritis Cartilage

Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease.

Authors - Jing Han
mouseIF

Cells

Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3.

Authors - David Baidoe-Ansah
mouseIHC

J Neurochem

OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex.

Authors - Hiroaki Okuda
humanIHC

Histochem Cell Biol

Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.

Authors - Jian Lei
humanWB

Biol Trace Elem Res

Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes.

Authors - Liyun Wang