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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CKB-Specific Polyclonal antibody

CKB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18713-1-AP

Synonyms

CKB, KCRB, Creatine kinase B-type, CPK-B, CKBB

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CKB-Specific Polyclonal antibody (18713-1-AP)

    WB analysis of HEK-293 using 18713-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CKB-Specific Polyclonal antibody (18713-1-AP)

    WB analysis of Y79 using 18713-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    WB analysis of mouse colon using 18713-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of mouse brain using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of mouse brain using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of mouse testis using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of mouse skeletal muscle using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of mouse skeletal muscle using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of human normal colon using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of human gliomas using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of human gliomas using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of human ovary tumor using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CKB-Specific Polyclonal antibody (18713-1-AP)

    IHC staining of human ovary tumor using 18713-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CKB-Specific Polyclonal antibody (18713-1-AP)

    IF Staining of HEK-293 using 18713-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18713-1-AP (CKB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse colon tissue, Y79 cells
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human gliomas tissue, mouse skeletal muscle tissue, mouse testis tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18713-1-AP targets CKB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name creatine kinase, brain
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001190
Gene symbol CKB
Gene ID (NCBI) 1152
RRIDAB_10596470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody is specific to CKB.

Protocols

Product Specific Protocols
WB protocol for CKB-Specific antibody 18713-1-APDownload protocol
IHC protocol for CKB-Specific antibody 18713-1-APDownload protocol
IF protocol for CKB-Specific antibody 18713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols