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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A2C6

Cat no : CL594-66903

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated Calnexin Monoclonal antibo (CL594-66903)

    WB analysis using CL594-66903

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66903 (Calnexin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CoraLite®594-conjugated Calnexin Monoclonal antibo (CL594-66903)

    IF Staining of SKOV-3 using CL594-66903

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Calnexin Monoclonal antibo (CL594-66903)

    IF Staining of HepG2 using CL594-66903

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66903 targets Calnexin in WB, IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calnexin fusion protein Ag25535 相同性解析による交差性が予測される生物種
Full Name calnexin
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC003552
Gene symbol Calnexin
Gene ID (NCBI) 821
RRIDAB_2920054
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
WB protocol for CL594 Calnexin antibody CL594-66903Download protocol
IF protocol for CL594 Calnexin antibody CL594-66903Download protocol
Standard Protocols
Click here to view our Standard Protocols