CD31 Polyclonal antibody

Name: CD31 Polyclonal antibody
Brand: Proteintech
SKU: 28083-1-AP

CD31 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 28083-1-AP

Synonyms

Pecam1, PECAM 1, Pecam

Validation Data Gallery

Filter:

AllWBIHCIF-P

WB analysis of bEnd.3 using 28083-1-AP
bEnd.3 cells were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 28083-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 28083-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28083-1-AP (Cd31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28083-1-AP (Cd31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 28083-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse liver using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse kidney using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD31 antibody (28083-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CD31 antibody (28083-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 28083-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CD31 antibody (28083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 28083-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	bEnd.3 cells, mouse liver tissue, mouse spleen tissue
Positive IHC detected in	mouse liver tissue, mouse brain tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue, mouse kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)-P	IF-P : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 27 publications below
IF	See 25 publications below
FC	See 1 publications below

Product Information

28083-1-AP targets CD31 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD31 fusion protein Ag27828 相同性解析による交差性が予測される生物種
Full Name	platelet/endothelial cell adhesion molecule 1
Calculated molecular weight	81 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	NM_001032378
Gene symbol	CD31
Gene ID (NCBI)	18613
RRID	AB_2881055
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 28083-1-AP	Download protocol
IHC protocol for CD31 antibody 28083-1-AP	Download protocol
IF protocol for CD31 antibody 28083-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IHC	Nanomicro Lett Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring Authors - Yuewei Chen View Article
mouse	FC	Cell Mol Biol Lett Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions Authors - Seyram Yao Adzraku View Article
mouse	IHC	Front Immunol Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis Authors - Fengxia Bai View Article
mouse	IF	Cells Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars Authors - Jorge Luis Montenegro Raudales View Article
mouse	IF	Front Immunol "γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy. Authors - Zhigang Zhang View Article
mouse	IHC	Appl Microbiol Biotechnol Recombinantly expressed rhFEB remodeled the skin defect of db/db mice Authors - Xiaomin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis of bEnd.3 using 28083-1-AP

WB analysis of mouse spleen using 28083-1-AP

WB analysis of mouse liver using 28083-1-AP

IHC staining of mouse liver using 28083-1-AP

IHC staining of mouse liver using 28083-1-AP

IHC staining of mouse spleen using 28083-1-AP

IHC staining of mouse spleen using 28083-1-AP

IHC staining of mouse brain using 28083-1-AP

IHC staining of mouse brain using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

IF Staining of mouse liver using 28083-1-AP

IF Staining of mouse liver using 28083-1-AP

IF Staining of mouse kidney using 28083-1-AP

IF Staining of mouse kidney using 28083-1-AP

IF Staining of mouse brain using 28083-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions

Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis

Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars

"γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy.

Recombinantly expressed rhFEB remodeled the skin defect of db/db mice