CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28083-1-AP

Synonyms

Pecam1, PECAM 1, Pecam


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Tested Applications

Positive WB detected inbEnd.3 cells, mouse liver tissue, mouse spleen tissue
Positive IHC detected inmouse liver tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28083-1-AP targets CD31 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD31 fusion protein Ag27828 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule 1
Calculated molecular weight 81 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberNM_001032378
Gene symbol CD31
Gene ID (NCBI) 18613
RRIDAB_2881055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 28083-1-APDownload protocol
IHC protocol for CD31 antibody 28083-1-APDownload protocol
IF protocol for CD31 antibody 28083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Nanomicro Lett

Gelatin-Based Metamaterial Hydrogel Films with High Conformality for Ultra-Soft Tissue Monitoring

Authors - Yuewei Chen
mouseFC

Cell Mol Biol Lett

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions

Authors - Seyram Yao Adzraku
mouseIHC

Front Immunol

Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis

Authors - Fengxia Bai
mouseIF

Cells

Dental Pulp Cell Transplantation Combined with Regenerative Endodontic Procedures Promotes Dentin Matrix Formation in Mature Mouse Molars

Authors - Jorge Luis Montenegro Raudales
mouseIF

Front Immunol

"γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy.

Authors - Zhigang Zhang
mouseIHC

Appl Microbiol Biotechnol

Recombinantly expressed rhFEB remodeled the skin defect of db/db mice

Authors - Xiaomin Li