Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 28459-1-AP

Synonyms

COL2, COL2A1, COL11A3, Chondrocalcin, AOM


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Tested Applications

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28459-1-AP targets Collagen Type II in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type II fusion protein Ag29265 相同性解析による交差性が予測される生物種
Full Name collagen, type II, alpha 1
Calculated molecular weight 134 kDa
GenBank accession numberBC007252
Gene symbol COL2A1
Gene ID (NCBI) 1280
RRIDAB_2881147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type II antibody 28459-1-APDownload protocol
IF protocol for Collagen Type II antibody 28459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
ratIF

Adv Mater

Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy

Authors - Manyu Chen
ratIF

Biomaterials

Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration

Authors - Lei Liu
humanWB

Nat Commun

Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.

Authors - Yun Zhang
humanWB

J Musculoskelet Neuronal Interact

Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway

Authors - Xiaoming Shi
rabbitIHC

Acta Biomater

The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage.

Authors - Wang Changchen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: colon Tissue