Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 14667-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 14667-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14667-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14667-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14667-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 14667-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14667-1-AP (Decorin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of zebrafish retina using 14667-1-AP
Immunofluorescence analysis of Decorin polyclonal antibody (14667-1-AP, magenta) staining on tissue sections of the zebrafish retina at 4 days post fertilisation (dpf). Dilution 1:200. Tissue fixed overnight in 4% PFA. DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.
IF Staining of mouse liver using 14667-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse liver using 14667-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of BxPC-3 using 14667-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using Decorin antibody (14667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Decorin Polyclonal antibody

Decorin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 14667-1-AP

Publications(56)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zabrafish and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

DCN, CSCD, DSPG2, PG S2, PG40

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (56)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells
Positive IHC detected in	human skin cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	zebrafish retina, mouse liver tissue
Positive IF/ICC detected in	BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 30 publications below
IHC	See 9 publications below
IF	See 19 publications below

Product Information

14667-1-AP targets Decorin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zabrafish samples.

Tested Reactivity	human, mouse, rat, zabrafish
Cited Reactivity	human, mouse, rat, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6328 Product name: Recombinant human DCN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC005322 Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK 相同性解析による交差性が予測される生物種
Full Name	decorin
Calculated molecular weight	359 aa, 40 kDa
Observed molecular weight	70 kDa, 45-48 kDa
GenBank accession number	BC005322
Gene Symbol	Decorin
Gene ID (NCBI)	1634
RRID	AB_2090265
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07585
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Protocols

Product Specific Protocols
IF protocol for Decorin antibody 14667-1-AP	Download protocol
IHC protocol for Decorin antibody 14667-1-AP	Download protocol
WB protocol for Decorin antibody 14667-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Epithelial cells activate fibroblasts to promote esophageal cancer development Authors - Yamei Chen View Article
human	IHC,IF	Nat Genet Spatially resolved transcriptomic analysis of the adult human prostate Authors - Junyi Hu View Article
		Cell Stem Cell Human iPSC-derived heart valve-like assembloids model valve development and disease pathology. Authors - Yuanhang He View Article
Human	IF	Bone Res CCN2 mediates fibroblast-macrophage interaction in knee arthrofibrosis based on single-cell RNA-seq analysis Authors - Ziyun Li View Article
human	IF	Nat Commun The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer Authors - Simeng Hu View Article
	IHC	Nat Commun Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors Authors - Chenhui Zhao View Article

Validation Data Gallery

WB analysis using 14667-1-AP

WB analysis of BxPC-3 using 14667-1-AP

WB analysis of mouse heart using 14667-1-AP

WB analysis of MCF-7 using 14667-1-AP

WB analysis of MCF-7 using 14667-1-AP

IHC staining of human colon using 14667-1-AP

IHC staining of human colon cancer using 14667-1-AP

IHC staining of human colon cancer using 14667-1-AP

IHC staining of human breast cancer using 14667-1-AP

IHC staining of human breast hyperplasia using 14667-1-AP

IHC staining of human breast hyperplasia using 14667-1-AP

IHC staining of human breast hyperplasia using 14667-1-AP

IHC staining of human skin cancer using 14667-1-AP

IHC staining of human skin cancer using 14667-1-AP

IHC staining of human skin cancer using 14667-1-AP

IF Staining of zebrafish retina using 14667-1-AP

IF Staining of mouse liver using 14667-1-AP

IF Staining of mouse liver using 14667-1-AP

IF Staining of BxPC-3 using 14667-1-AP

Decorin Polyclonal antibody

Decorin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Epithelial cells activate fibroblasts to promote esophageal cancer development

Spatially resolved transcriptomic analysis of the adult human prostate

Human iPSC-derived heart valve-like assembloids model valve development and disease pathology.

CCN2 mediates fibroblast-macrophage interaction in knee arthrofibrosis based on single-cell RNA-seq analysis

The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer

Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors

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