Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 25420-1-AP
WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells.
WB analysis of MCF-7 using 25420-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 25420-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25420-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25420-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25420-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 25420-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25420-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 25420-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DKC1 Polyclonal antibody

DKC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25420-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

25420-1-AP targets DKC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DKC1 fusion protein Ag7992 相同性解析による交差性が予測される生物種
Full Name	dyskeratosis congenita 1, dyskerin
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	BC009928
Gene Symbol	DKC1
Gene ID (NCBI)	1736
RRID	AB_2880071
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60832
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DKC1 antibody 25420-1-AP	Download protocol
IHC protocol for DKC1 antibody 25420-1-AP	Download protocol
IF protocol for DKC1 antibody 25420-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Stem Cells H/ACA Box Small Nucleolar RNA 7A promotes the Self-Renewal of Human Umbilical Cord Mesenchymal Stem Cells. Authors - Yan Zhang View Article
human	WB	Cell Rep Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. Authors - Cai Han KD Validated View Article
human	WB,IHC,IF	J Cancer Res Clin Oncol Identifying and validating the roles of the cuproptosis-related gene DKC1 in cancer with a focus on esophageal carcinoma Authors - Daidi Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 25420-1-AP

WB analysis of MCF-7 using 25420-1-AP

WB analysis of COLO 320 using 25420-1-AP

WB analysis of HeLa using 25420-1-AP

WB analysis of HepG2 using 25420-1-AP

WB analysis of Jurkat using 25420-1-AP

IHC staining of human colon cancer using 25420-1-AP