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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT1 Polyclonal antibody

DNMT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28754-1-AP

Synonyms

EC:2.1.1.37, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT1 Polyclonal antibody (28754-1-AP)

    WB analysis using 28754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28754-1-AP (DNMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293T cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28754-1-AP targets DNMT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNMT1 fusion protein Ag29479 相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight 1632 aa, 185 kDa
Observed molecular weight145 kDa and 184 kDa
GenBank accession numberBC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with the molecular mass of 184 kDa and 145 kDa. (PMID: 10753866)

Protocols

Product Specific Protocols
WB protocol for DNMT1 antibody 28754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols