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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Coagulation Factor II Monoclonal antibody (66509-1-Ig)

    WB analysis of human plasma using 66509-1-Ig

    human plasma was subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at various dilutions incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Coagulation Factor II Monoclonal antibody (66509-1-Ig)

    WB analysis of human plasma using 66509-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Immunohistochemistry (IHC) staining of human liver tissue using Coagulation Factor II Monoclonal antibody (66509-1-Ig)

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Coagulation Factor II Monoclonal antibody (66509-1-Ig)

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 40x lens).

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Coagulation Factor II Monoclonal antibody

Coagulation Factor II Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66509-1-Ig
Clone No.1E12A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Prothrombin, F2, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66509-1-Ig targets Coagulation Factor II in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Coagulation Factor II fusion protein Ag18385 相同性解析による交差性が予測される生物種
Full Name coagulation factor II (prothrombin)
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC051332
Gene Symbol F2
Gene ID (NCBI) 2147
RRIDAB_2881872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

Protocols

Product Specific Protocols
WB protocol for Coagulation Factor II antibody 66509-1-IgDownload protocol
IHC protocol for Coagulation Factor II antibody 66509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols