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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F2 Monoclonal antibody

F2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12A8

Cat no : 66509-1-Ig

Synonyms

Coagulation factor II, Prothrombin, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using F2 Monoclonal antibody (66509-1-Ig)

    WB analysis of human plasma using 66509-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (F2 antibody) at various dilutions incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using F2 Monoclonal antibody (66509-1-Ig)

    WB analysis of human plasma using 66509-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (F2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Immunohistochemistry (IHC) staining of human liver tissue using F2 Monoclonal antibody (66509-1-Ig)

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (F2 antibody) at dilution of 1:150 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using F2 Monoclonal antibody (66509-1-Ig)

    IHC staining of human liver using 66509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (F2 antibody) at dilution of 1:150 (under 40x lens).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66509-1-Ig targets F2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen F2 fusion protein Ag18385 相同性解析による交差性が予測される生物種
Full Name coagulation factor II (prothrombin)
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC051332
Gene symbol F2
Gene ID (NCBI) 2147
RRIDAB_2881872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

Protocols

Product Specific Protocols
WB protocol for F2 antibody 66509-1-IgDownload protocol
IHC protocol for F2 antibody 66509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols