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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F8 Polyclonal antibody

F8 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 21485-1-AP

Synonyms

AHF, Antihemophilic factor, Coagulation factor VIII, DXS1253E, F8, F8B, F8C, FVIII, HEMA, Procoagulant component

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human umbilical vein endothelial cells using F8 Polyclonal antibody (21485-1-AP)

    IF Staining of human umbilical vein endothelial cells using 21485-1-AP

    IF result of anti-F8 (21485-1-AP) with primary human umbilical vein endothelial cells.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using F8 Polyclonal antibody (21485-1-AP)

    IF Staining of HUVEC using 21485-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 21485-1-AP (F8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IF/ICC detected inhuman umbilical vein endothelial cells, HUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : N/A
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 21485-1-AP is F8 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F8 fusion protein Ag15772 相同性解析による交差性が予測される生物種
Full Name coagulation factor VIII, procoagulant component
Calculated molecular weight 2351 aa, 267 kDa
GenBank accession numberBC022513
Gene symbol F8
Gene ID (NCBI) 2157
RRIDAB_2878868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.

Protocols

Product Specific Protocols
IF protocol for F8 antibody 21485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Invest Ophthalmol Vis Sci

miR-590-3p Inhibits Pyroptosis in Diabetic Retinopathy by Targeting NLRP1 and Inactivating the NOX4 Signaling Pathway.

Authors - Chufeng Gu
View Article