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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXL14 Polyclonal antibody

FBXL14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13934-1-AP

Synonyms

F-box/LRR-repeat protein 14, F-box and leucine-rich repeat protein 14, FBL14

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FBXL14 Polyclonal antibody (13934-1-AP)

    WB analysis of HepG2 using 13934-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FBXL14 Polyclonal antibody (13934-1-AP)

    WB analysis of HepG2 using 13934-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FBXL14 Polyclonal antibody (13934-1-AP)

    WB analysis of HepG2 using 13934-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13934-1-AP (FBXL14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using FBXL14 Polyclonal antibody (13934-1-AP)

    IHC staining of human liver using 13934-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FBXL14 Polyclonal antibody (13934-1-AP)

    IHC staining of human liver using 13934-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBXL14 Polyclonal antibody (13934-1-AP)

    IF Staining of HepG2 using 13934-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13934-1-AP (FBXL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13934-1-AP targets FBXL14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL14 fusion protein Ag4975 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 14
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC028132
Gene symbol FBXL14
Gene ID (NCBI) 144699
RRIDAB_10547838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572).

Protocols

Product Specific Protocols
WB protocol for FBXL14 antibody 13934-1-APDownload protocol
IHC protocol for FBXL14 antibody 13934-1-APDownload protocol
IF protocol for FBXL14 antibody 13934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

Cell Death Dis

F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7.

Authors - Y Liu
View Article
humanWB

Commun Biol

The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells

Authors - Christopher Noyes
View Article