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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO18 Polyclonal antibody

FBXO18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14275-1-AP

Synonyms

FBX18, F-box only protein 18, F-box DNA helicase 1, FBH1, EC:5.6.2.4

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FBXO18 Polyclonal antibody (14275-1-AP)

    WB analysis of PC-3 using 14275-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FBXO18 Polyclonal antibody (14275-1-AP)

    WB analysis of HeLa using 14275-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FBXO18 Polyclonal antibody (14275-1-AP)

    WB analysis of HeLa using 14275-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14275-1-AP (FBXO18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using FBXO18 Polyclonal antibody (14275-1-AP)

    IF Staining of PC-3 using 14275-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 14275-1-AP (FBXO18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, HeLa cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14275-1-AP targets FBXO18 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO18 fusion protein Ag5622 相同性解析による交差性が予測される生物種
Full Name F-box protein, helicase, 18
Calculated molecular weight 118 kDa, 123 kDa
Observed molecular weight 118-123 kDa
GenBank accession numberBC032674
Gene symbol FBXO18
Gene ID (NCBI) 84893
RRIDAB_10638916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FBXO18 antibody 14275-1-APDownload protocol
IF protocol for FBXO18 antibody 14275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols