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  • KD/KO Validated

FBXO22 Polyclonal antibody

FBXO22 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13606-1-AP

Synonyms

F box only protein 22, F box protein 22, F box protein FBX22p44, FBX22, FBXO22, FISTC1



Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13606-1-AP targets FBXO22 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO22 fusion protein Ag4485 相同性解析による交差性が予測される生物種
Full Name F-box protein 22
Calculated molecular weight 403 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC039024
Gene symbol FBXO22
Gene ID (NCBI) 26263
RRIDAB_2104403
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Protocols

Product Specific Protocols
WB protocol for FBXO22 antibody 13606-1-APDownload protocol
IHC protocol for FBXO22 antibody 13606-1-APDownload protocol
IP protocol for FBXO22 antibody 13606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell

Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.

Authors - Luca Lignitto
  • KO Validated
humanWB

Nat Commun

Targeted inhibition of the COP9 signalosome for treatment of cancer.

Authors - Anita Schlierf
humanWB

Cell Death Differ

Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.

Authors - Ping Liu
  • KO Validated
humanWB

Front Oncol

Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.

Authors - Sen Chen
humanWB

iScience

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

Authors - Muzammal Hussain
humanWB,IHC

Front Pharmacol

FBXO22 Promotes Growth and Metastasis and Inhibits Autophagy in Epithelial Ovarian Cancers via the MAPK/ERK Pathway.

Authors - Minle Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Strong single band at predicted size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart