Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HuH-7 cells, Jurkat cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 13 publications below |
| WB | See 28 publications below |
| IHC | See 11 publications below |
| IF | See 2 publications below |
| IP | See 3 publications below |
Product Information
13606-1-AP targets FBXO22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXO22 fusion protein Ag4485 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 22 |
| Calculated molecular weight | 403 aa, 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC039024 |
| Gene Symbol | FBXO22 |
| Gene ID (NCBI) | 26263 |
| RRID | AB_2104403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO22 antibody 13606-1-AP | Download protocol |
| IHC protocol for FBXO22 antibody 13606-1-AP | Download protocol |
| IP protocol for FBXO22 antibody 13606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress | ||
Cell Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.
| ||
Cell Death Differ Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.
| ||
Cell Death Dis Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis
| ||
Front Oncol Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22. |