FBXO28 Polyclonal antibody
FBXO28 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24282-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
The immunogen of 24282-1-AP is FBXO28 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXO28 fusion protein Ag14900 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 28 |
| Calculated molecular weight | 368 aa, 41 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC031576 |
| Gene symbol | FBXO28 |
| Gene ID (NCBI) | 23219 |
| RRID | AB_2879469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO28 also named as F box only protein 28 is a 368 amino-acid protein, which contain 1 F-box domain and belongs to F-box protein family. FBXO28 is a critical component of SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. FBOX28 encodes a cell cycle regulated protein with a critical function in tumor cell proliferation. FBXO28 assembles an SCF-FBXO28 ubiquitin ligase whose activity is required during progression through the cell cycle by cyclin-dependent kinase 1/2 mediated phosphorylation. FBXO28 expression and phosphorylation to be strongly associated with poor prognosis and worse overall survival in human breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| IHC protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation |
