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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO43 Polyclonal antibody

FBXO43 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55176-1-AP

Synonyms

EMI2, Endogenous meiotic inhibitor 2, ERP1, F box only protein 43, F box protein 43, Fbp5B, Fbx43, FBXO43

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 55176-1-AP

    WB analysis of HEK-293 using 55176-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55176-1-AP (FBXO43 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 55176-1-AP

    IF Staining of HEK-293 using 55176-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 55176-1-AP (FBXO43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55176-1-AP targets FBXO43 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name F-box protein 43
Calculated molecular weight 78 kDa
Observed molecular weight ~70 kDa
GenBank accession numberNM_001029860
Gene symbol FBXO43
Gene ID (NCBI) 286151
RRIDAB_2881282
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO43, also named as EMI2 and FBP5B, is required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. It acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. FBXO43 recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. We got ~70 kDa band in our detection.

Protocols

Product Specific Protocols
WB protocol for FBXO43 antibody 55176-1-APDownload protocol
IF protocol for FBXO43 antibody 55176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

SKP1 drives the prophase I to metaphase I transition during male meiosis.

Authors - Yongjuan Guan
View Article
humanWB,IHC

Cancers (Basel)

F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

Authors - Huijun Zhou
  • KD Validated
View Article
humanWB,IHC

Front Oncol

FBXO43 increases CCND1 stability to promote hepatocellular carcinoma cell proliferation and migration

Authors - Chun-Ming Li
View Article