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- KD/KO Validated
FBXO43 Polyclonal antibody
FBXO43 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55176-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
55176-1-AP targets FBXO43 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 43 |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | ~70 kDa |
| GenBank accession number | NM_001029860 |
| Gene symbol | FBXO43 |
| Gene ID (NCBI) | 286151 |
| RRID | AB_2881282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO43, also named as EMI2 and FBP5B, is required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. It acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. FBXO43 recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. We got ~70 kDa band in our detection.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO43 antibody 55176-1-AP | Download protocol |
| IF protocol for FBXO43 antibody 55176-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2
| ||
Cancers (Basel) F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner
| ||
Front Oncol FBXO43 increases CCND1 stability to promote hepatocellular carcinoma cell proliferation and migration |
