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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO6 Polyclonal antibody

FBXO6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 11830-1-AP

Synonyms

F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO6 Polyclonal antibody (11830-1-AP)

    WB analysis using 11830-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11830-1-AP (FBXO6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FBXO6 Polyclonal antibody (11830-1-AP)

    IHC staining of human prostate cancer using 11830-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11830-1-AP (FBXO6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FBXO6 Polyclonal antibody (11830-1-AP)

    IHC staining of human prostate cancer using 11830-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11830-1-AP (FBXO6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11830-1-AP targets FBXO6 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO6 fusion protein Ag2400 相同性解析による交差性が予測される生物種
Full Name F-box protein 6
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC020880
Gene symbol FBXO6
Gene ID (NCBI) 26270
RRIDAB_2104709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs.(PMID:19716789). FBXO6 can inhibit the anaphase promoting complex (cyclosome APC) and prevent mitotic exit in cytostatic factor (CSF) arrested eggs as a mitotic regulator.

Protocols

Product Specific Protocols
WB protocol for FBXO6 antibody 11830-1-APDownload protocol
IHC protocol for FBXO6 antibody 11830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Cell Signal

Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis

Authors - Tao Zhang
View Article