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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXO7 Polyclonal antibody

FBXO7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10696-1-AP

Synonyms

PARK15, FBXO 7, FBX7, FBX07, FBX

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO7 Polyclonal antibody (10696-1-AP)

    WB analysis using 10696-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10696-1-AP (FBXO7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FBXO7 Polyclonal antibody (10696-1-AP)

    IHC staining of human stomach using 10696-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10696-1-AP (FBXO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FBXO7 Polyclonal antibody (10696-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10696-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10696-1-AP (FBXO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10696-1-AP targets FBXO7 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO7 fusion protein Ag1035 相同性解析による交差性が予測される生物種
Full Name F-box protein 7
Calculated molecular weight 59 kDa
Observed molecular weight 59-75 kDa
GenBank accession numberBC008361
Gene symbol FBXO7
Gene ID (NCBI) 25793
RRIDAB_2246820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.

Protocols

Product Specific Protocols
WB protocol for FBXO7 antibody 10696-1-APDownload protocol
IHC protocol for FBXO7 antibody 10696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Hepatology

Novel mechanism by which histone deacetylase inhibitors facilitate topoisomerase IIα degradation in hepatocellular carcinoma cells.

Authors - Chen Mei-Chuan MC
View Article
human

JCI Insight

Chemical inhibition of FBXO7 reduces inflammation and confers neuroprotection by stabilizing the mitochondrial kinase PINK1.

Authors - Yuan Liu
View Article