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FBXW11 Polyclonal antibody

FBXW11 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13149-1-AP

Synonyms

BTRC2, BTRCP2, Fbw11, FBW1B, FBXW11, FBXW1B, Homologous to Slimb protein, Hos, KIAA0696



Tested Applications

Positive WB detected inA431 cells, SGC-7901 cells, human stomach tissue, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHCT 116 cells, A431 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

13149-1-AP targets FBXW11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW11 fusion protein Ag3761 相同性解析による交差性が予測される生物種
Full Name F-box and WD repeat domain containing 11
Calculated molecular weight 542 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC026213
Gene symbol FBXW11
Gene ID (NCBI) 23291
RRIDAB_2102595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.

Protocols

Product Specific Protocols
WB protocol for FBXW11 antibody 13149-1-APDownload protocol
IHC protocol for FBXW11 antibody 13149-1-APDownload protocol
IF protocol for FBXW11 antibody 13149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.

Authors - Jie Xu
humanWB

Cell Death Differ

NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.

Authors - Hao Li
humanWB,IP

EMBO J

The E3 ubiquitin ligase APC/CCdh1 degrades MCPH1 after MCPH1-βTrCP2-Cdc25A-mediated mitotic entry to ensure neurogenesis.

Authors - Xiaoqian Liu
WB

Antioxidants (Basel)

AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its βTrCP2-Mediated Degradation in KEAP1-Deficient Cells

Authors - Eleni Petsouki
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H
humanWB

Front Oncol

uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.

Authors - Zhijian Zheng