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FBXW11 Polyclonal antibody

Name: FBXW11 Polyclonal antibody
Brand: Proteintech
SKU: 13149-1-AP

FBXW11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13149-1-AP

Synonyms

FBW1B, Fbw11, F-box/WD repeat-containing protein 1B, F-box/WD repeat-containing protein 11, F-box and WD repeats protein beta-TrCP2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 13149-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13149-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 13149-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 13149-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 13149-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 13149-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 13149-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 13149-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human stomach cancer using 13149-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HCT 116 using 13149-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A431 using 13149-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of U2OS using 13149-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13149-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, SGC-7901 cells, human stomach tissue, A549 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HCT 116 cells, A431 cells, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

13149-1-AP targets FBXW11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FBXW11 fusion protein Ag3761 相同性解析による交差性が予測される生物種
Full Name	F-box and WD repeat domain containing 11
Calculated molecular weight	542 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC026213
Gene symbol	FBXW11
Gene ID (NCBI)	23291
RRID	AB_2102595
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.

Protocols

Product Specific Protocols
WB protocol for FBXW11 antibody 13149-1-AP	Download protocol
IHC protocol for FBXW11 antibody 13149-1-AP	Download protocol
IF protocol for FBXW11 antibody 13149-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor. Authors - Jie Xu View Article
human	WB	Cell Death Differ NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex. Authors - Hao Li View Article
human	WB,IP	EMBO J The E3 ubiquitin ligase APC/CCdh1 degrades MCPH1 after MCPH1-βTrCP2-Cdc25A-mediated mitotic entry to ensure neurogenesis. Authors - Xiaoqian Liu View Article
	WB	Antioxidants (Basel) AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its βTrCP2-Mediated Degradation in KEAP1-Deficient Cells Authors - Eleni Petsouki View Article
human	WB	Mol Cell Biol DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase. Authors - Chen Hao H View Article
human	WB	Front Oncol uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation. Authors - Zhijian Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXW11 Polyclonal antibody

FBXW11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 13149-1-AP

WB analysis using 13149-1-AP

WB analysis using 13149-1-AP

WB analysis using 13149-1-AP

WB analysis of SGC-7901 using 13149-1-AP

WB analysis of human stomach using 13149-1-AP

WB analysis of SGC-7901 using 13149-1-AP

IHC staining of human stomach cancer using 13149-1-AP

IHC staining of human stomach cancer using 13149-1-AP

IF Staining of HCT 116 using 13149-1-AP

IF Staining of A431 using 13149-1-AP

IF Staining of U2OS using 13149-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.

NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.

The E3 ubiquitin ligase APC/CCdh1 degrades MCPH1 after MCPH1-βTrCP2-Cdc25A-mediated mitotic entry to ensure neurogenesis.

AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its βTrCP2-Mediated Degradation in KEAP1-Deficient Cells

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.