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FBXW2 Polyclonal antibody
FBXW2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 11499-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
11499-1-AP targets FBXW2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXW2 fusion protein Ag2045 相同性解析による交差性が予測される生物種 |
| Full Name | F-box and WD repeat domain containing 2 |
| Calculated molecular weight | 44 kDa, 52 kDa |
| Observed molecular weight | 52 kDa, 43 kDa |
| GenBank accession number | BC018738 |
| Gene symbol | FBXW2 |
| Gene ID (NCBI) | 26190 |
| RRID | AB_2102740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| IHC protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
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Cell Mol Gastroenterol Hepatol TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.
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Cell Mol Life Sci FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradation.
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Nat Commun FBXW2 suppresses migration and invasion of lung cancer cells via promoting β-catenin ubiquitylation and degradation.
| ||
Cell Death Dis FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis
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