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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FOXO4 Polyclonal antibody

FOXO4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19121-1-AP

Synonyms

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using FOXO4 Polyclonal antibody (19121-1-AP)

    WB analysis of human heart using 19121-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19121-1-AP (FOXO4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FOXO4 Polyclonal antibody (19121-1-AP)

    WB analysis of mouse heart using 19121-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19121-1-AP (FOXO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FOXO4 Polyclonal antibody (19121-1-AP)

    IF Staining of HepG2 using 19121-1-AP

    Immunofluorescent analysis of HepG2 cells, using FOXO4 antibody 19121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman heart tissue, mouse heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19121-1-AP targets FOXO4 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name forkhead box O4
Calculated molecular weight 54 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberNM_005938
Gene symbol FOXO4
Gene ID (NCBI) 4303
RRIDAB_2106957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the ins signaling pathway. It binds to ins-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Protocols

Product Specific Protocols
WB protocol for FOXO4 antibody 19121-1-APDownload protocol
IF protocol for FOXO4 antibody 19121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Neurosci Lett

Expression of FOXO transcription factors in the brain following traumatic brain injury.

Authors - Xi-Lin Liu
View Article