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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Factor XIIIa Monoclonal antibody

Factor XIIIa Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2B2

Cat no : 66325-1-Ig

Synonyms

F13A1, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13, Coagulation factor XIIIa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    WB analysis using 66325-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    WB analysis of human peripheral blood platelets using 66325-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit large intestine tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    WB analysis of rabbit large intestine using 66325-1-Ig

    rabbit large intestine tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    WB analysis of human placenta using 66325-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    WB analysis of rat colon using 66325-1-Ig

    rat colon tissue was subjected to SDS PAGE followed by western blot with 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    IHC staining of human liver cancer using 66325-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    IHC staining of human liver cancer using 66325-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66325-1-Ig (Factor XIIIA antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    IHC staining of human endometrial cancer using 66325-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Factor XIIIa Monoclonal antibody (66325-1-Ig)

    IHC staining of human endometrial cancer using 66325-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66325-1-Ig (Factor XIIIa antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig colon tissue, human placenta tissue, human peripheral blood platelets, rat colon tissue, rabbit large intestine tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66325-1-Ig targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XIIIa fusion protein Ag11105 相同性解析による交差性が予測される生物種
Full Name coagulation factor XIII, A1 polypeptide
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC027963
Gene symbol Factor XIIIa
Gene ID (NCBI) 2162
RRIDAB_2881706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor , and meningioma.

Protocols

Product Specific Protocols
WB protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
IHC protocol for Factor XIIIa antibody 66325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Inflamm Regen

Expression of factor XIII originating from synovial fibroblasts and macrophages induced by interleukin-6 signaling

Authors - Hirofumi Watanabe
View Article