DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)
DDDDK tag Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
recombinant protein and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1C1D2
Cat no : 60002-1-Ig
Synonyms
"DDDDK tag Antibodies" Comparison
View side-by-side comparison of DDDDK tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | Recombinant Protein |
Positive IP detected in | Recombinant protein protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IP | See 3 publications below |
Product Information
60002-1-Ig targets DDDDK tag in WB, IP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Reactivity | recombinant protein |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | DDDDK tag |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.
Protocols
Product Specific Protocols | |
---|---|
IP protocol for DDDDK tag antibody 60002-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Aging (Albany NY) HOXB9 enhances the ability of lung cancer cells to penetrate the blood-brain barrier. | ||
Cell Death Dis TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability. | ||
Curr Microbiol Host filamentous actin is associated with Heliothis armigera single nucleopolyhedrosis virus (HaSNPV) nucleocapsid transport to the host nucleus. | ||
Biol Open ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane. | ||
Mol Cell Biochem INMAP, a novel truncated version of POLR3B, represses AP-1 and p53 transcriptional activity. | ||