Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 15862-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 15862-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 15862-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 15862-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 15862-1-AP
IP result of anti-HLA-DRB1 (IP:15862-1-AP, 4ug; Detection:15862-1-AP 1:500) with Raji cells lysate 2000ug.
IHC staining of human tonsillitis using 15862-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 15862-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Raji using 15862-1-AP
Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB1 antibody (15862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15862-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Raji cells, human spleen tissue, A375 cells, Daudi cells
Positive IP detected in	Raji cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HLA-DRB1 fusion protein Ag8405 相同性解析による交差性が予測される生物種
Full Name	major histocompatibility complex, class II, DR beta 1
Calculated molecular weight	266 aa, 30 kDa
Observed molecular weight	27-30 kDa
GenBank accession number	BC007920
Gene Symbol	HLA-DRB1
Gene ID (NCBI)	3123
RRID	AB_1639897
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01911
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IHC protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IF protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol
IP protocol for HLA-DRB1 antibody 15862-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biochem Pharmacol Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation. Authors - Semini Geo G View Article
human	WB	Nat Commun Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes Authors - Rupert L Mayer View Article
mouse	WB	Heliyon Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation Authors - Qian Gao View Article
human	IHC	Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis Authors - Yanling Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15862-1-AP

WB analysis of A375 using 15862-1-AP

WB analysis of Raji using 15862-1-AP

WB analysis of human spleen using 15862-1-AP

IP experiment of Raji using 15862-1-AP

IHC staining of human tonsillitis using 15862-1-AP

IHC staining of human tonsillitis using 15862-1-AP

IF Staining of Raji using 15862-1-AP

HLA-DRB1 Polyclonal antibody

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.

Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes

Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis