Human CXCL4/PF4 ELISA Kit
Sensitivity
0.08 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00257
Synonyms
C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4
Validation Data Gallery
Product Information
KE00257 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human CXCL4/PF4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CXCL4/PF4. The assay recognizes human CXCL4/PF4. An antibody specific for human CXCL4/PF4 has been pre-coated onto the microwells. The human CXCL4/PF4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL4/PF4 is added to detect the captured human CXCL4/PF4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL4/PF4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.08 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5196 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88% | 74%-100% |
| Cell culture supernatants | 99% | 88%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 486.9 | 19.9 | 4.1 |
| 2 | 20 | 110.7 | 6.1 | 5.5 |
| 3 | 20 | 26.3 | 1.2 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 504.2 | 16.4 | 3.3 |
| 2 | 24 | 116.1 | 3.6 | 3.1 |
| 3 | 24 | 28.9 | 2.3 | 7.9 |
Background Information
CXCL4, alternatively known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4 |