Human EPO ELISA Kit
Cat no : KE00153
Synonyms
EP, EPO, Epoetin, erythropoietin, MVCD2
Validation Data Gallery
Product Information
KE00153 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Erythropoietin ELISA kit is to be used to detect and quantify protein levels of endogenous Erythropoietin. The assay recognizes mouse Erythropoietin. An antibody specific for Erythropoietin has been pre-coated onto the microwells. The Erythropoietin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Erythropoietin is added to detect the captured mouse Erythropoietin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human EPO ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.2 mIU/mL |
| Range | 4.7-300 mIU/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2056 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 71-107% |
| Human plasma | 92% | 72-106% |
IntraAssay
| Sample | n | mean ( mIU/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 7.0 | 0.54 | 7.7 |
| 2 | 20 | 32.2 | 1.10 | 3.4 |
| 3 | 20 | 137.4 | 3.99 | 2.9 |
InterAssay
| Sample | n | mean ( mIU/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7.5 | 0.61 | 8.2 |
| 2 | 24 | 31.9 | 2.94 | 9.2 |
| 3 | 24 | 129.2 | 13.59 | 10.5 |
Background Information
Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. Low levels of EPO (around 10 mIU/mL) are constantly secreted sufficient to compensate for normal red blood cell turnover. Common causes of cellular hypoxia resulting in elevated levels of EPO (up to 10000 mIU/mL) include any anemia, and hypoxemia due to chronic lung disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EP, EPO, Epoetin, erythropoietin, MVCD2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Invest Multi-organ failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. |