Human Vitamin D BP ELISA Kit
Sensitivity
0.005 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00272
Synonyms
DBP, DBP/GC, GC, Gc globulin, Group specific component, VDB, VDBG, VDBP, Vitamin D binding protein
Validation Data Gallery
Product Information
KE00272 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Vitamin D BP ELISA kit is to be used to detect and quantify protein levels of endogenous human Vitamin D BP. The assay recognizes human Vitamin D BP. An antibody specific for human Vitamin D BP has been pre-coated onto the microwells. The human Vitamin D BP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Vitamin D BP is added to detect the captured human Vitamin D BP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Vitamin D BP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.005 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2638 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 90%-112% |
| Human urine | 75% | 71%-81% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.04 | 0.11 | 2.85 |
| 2 | 20 | 1.02 | 0.03 | 2.88 |
| 3 | 20 | 0.27 | 0.01 | 5.46 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.84 | 0.14 | 3.67 |
| 2 | 24 | 0.99 | 0.02 | 2.05 |
| 3 | 24 | 0.26 | 0.02 | 6.49 |
Background Information
Vitamin D BP belongs to the albumin family and it is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It is expressed in the liver. And it is involved in vitamin D transport and storage, scavenges of extracellular G-actin, and enhances of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation. The presence of genetic variants in the Vitamin D BP gene are related to certain diseases, mostly concerning cancers (breast, prostate, pancreatic, lung, colorectal, basal cell carcinoma cancer and cutaneous melanoma) or other related diseases (thyroid autoimmunity disorders, obesity, diabetes mellitus, bone metabolism, rheumatoid arthritis, ankylosing spondylitis, asthma, chronic obstructive pulmonary disease, tuberculosis and coronary artery diseases).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DBP, DBP/GC, GC, Gc globulin, Group specific component, VDB, VDBG, VDBP, Vitamin D binding protein |