IL-1RA Polyclonal antibody

Name: IL-1RA Polyclonal antibody
Brand: Proteintech
SKU: 10844-1-AP

IL-1RA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10844-1-AP

Synonyms

IL 1ra, IL1RN, IL-1RN, IL1RA, IL 1RN

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 10844-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 10844-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).
IHC staining of human oesophagus using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).
IHC staining of human stomach using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).
IHC staining of human stomach using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon cancer using 10844-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10844-1-AP (IL1RA antibody) at dilution of 1:100 (under 10x lens).
IF Staining of A431 using 10844-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RA antibody (10844-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of A431 using 10844-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human IL-1RA (10844-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10844-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, MOLT-4 cells, RAW 264.7 cells
Positive IHC detected in	human oesophagus tissue, human colon tissue, human colon cancer tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10844-1-AP targets IL-1RA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IL-1RA fusion protein Ag1277 相同性解析による交差性が予測される生物種
Full Name	interleukin 1 receptor antagonist
Calculated molecular weight	20 kDa
Observed molecular weight	18-20 kDa
GenBank accession number	BC009745
Gene symbol	IL-1RA
Gene ID (NCBI)	3557
RRID	AB_2125559
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-1RA antibody 10844-1-AP	Download protocol
IHC protocol for IL-1RA antibody 10844-1-AP	Download protocol
IF protocol for IL-1RA antibody 10844-1-AP	Download protocol
FC protocol for IL-1RA antibody 10844-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Clin Transl Oncol Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells. Authors - J Zhang View Article
human	WB	NeuroImmune Pharm Ther Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages Authors - Santosh Kumar View Article
human	IHC	Breast Cancer (Dove Med Press) Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer Authors - Shen Ma View Article
mouse	IF	Front Immunol The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy Authors - Yanli Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1RA Polyclonal antibody

IL-1RA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10844-1-AP

WB analysis using 10844-1-AP

IHC staining of human oesophagus using 10844-1-AP

IHC staining of human oesophagus using 10844-1-AP

IHC staining of human stomach using 10844-1-AP

IHC staining of human colon using 10844-1-AP

IHC staining of human stomach using 10844-1-AP

IHC staining of human stomach using 10844-1-AP

IHC staining of human colon using 10844-1-AP

IHC staining of human colon cancer using 10844-1-AP

IF Staining of A431 using 10844-1-AP

FC experiment of A431 using 10844-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.

Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages

Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer

The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy