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Integrin alpha 6 Polyclonal antibody

Name: Integrin alpha 6 Polyclonal antibody
Brand: Proteintech
SKU: 27189-1-AP
Rating: 5.00 (1 reviews)

Integrin alpha 6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 27189-1-AP

Synonyms

ITGA6, integrin, alpha 6, Integrin alpha-6 light chain, Integrin alpha-6 heavy chain, Integrin alpha-6

Validation Data Gallery

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AllWBIHC

WB analysis of A549 using 27189-1-AP
WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.
WB analysis using 27189-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27189-1-AP
WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.
WB analysis of A431 using 27189-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 27189-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 27189-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skin using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 27189-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 27189-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, A431 cells, mouse skin tissue, rat lung tissue, HepG2 cells
Positive IHC detected in	mouse skin tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 6 publications below
IF	See 7 publications below

Product Information

27189-1-AP targets Integrin alpha 6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Integrin alpha 6 fusion protein Ag25835 相同性解析による交差性が予測される生物種
Full Name	integrin, alpha 6
Calculated molecular weight	1073 aa, 119 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	BC136456
Gene symbol	Integrin alpha 6
Gene ID (NCBI)	3655
RRID	AB_2880792
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 6 antibody 27189-1-AP	Download protocol
IHC protocol for Integrin alpha 6 antibody 27189-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	ACS Appl Mater Interfaces A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells Authors - Xinyang Zhao View Article
bovine	IF	Cells Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak Authors - Xiaolan Zhang View Article
monkey	WB	Virulence Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection. Authors - Yuchen Li View Article
human	WB	Stem Cell Res Ther Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Authors - Ling Leng View Article
Hamster	IF	J Virol Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus. Authors - Xiu Xin View Article
rat	IF	Vascul Pharmacol Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6 Authors - Li Wu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

Applications: Other
Primary Antibody Dilution: 1:100-1:400
Cell Tissue Type: iPSC derived Astrocytes

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin alpha 6 Polyclonal antibody

Integrin alpha 6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of A549 using 27189-1-AP

WB analysis using 27189-1-AP

WB analysis of HepG2 using 27189-1-AP

WB analysis of A431 using 27189-1-AP

WB analysis of mouse skin using 27189-1-AP

WB analysis of rat lung using 27189-1-AP

IHC staining of mouse skin using 27189-1-AP

IHC staining of mouse skin using 27189-1-AP

IHC staining of human colon using 27189-1-AP

IHC staining of human colon using 27189-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak

Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6

Reviews

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