Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, mouse colon tissue, A549 cells, Hela cells, mouse spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 48 publications below |
| IHC | See 4 publications below |
| IF | See 10 publications below |
| IP | See 8 publications below |
| CoIP | See 2 publications below |
| RIP | See 2 publications below |
Product Information
10723-1-AP targets KU70/XRCC6 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1043 Product name: Recombinant human KU70,XRCC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 307-609 aa of BC008343 Sequence: TGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD 相同性解析による交差性が予測される生物種 |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC008343 |
| Gene Symbol | KU70 |
| Gene ID (NCBI) | 2547 |
| RRID | AB_2218756 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12956 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IF protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IHC protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| IP protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| WB protocol for KU70/XRCC6 antibody 10723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma | ||
Cancer Res DNA-PK-Mediated Cytoplasmic DNA Sensing Stimulates Glycolysis to Promote Lung Squamous Cell Carcinoma Malignancy and Chemoresistance | ||
Drug Resist Updat Targeting CLK1/SRSF7 axis-dependent alternative splicing sensitizes pancreatic ductal adenocarcinoma to chemotherapy and immunotherapy | ||
Nat Commun Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability | ||
Nat Commun Systematic discovery of UFM1 receptors reveals a regulatory module in DNA repair directing non-homologous end-joining. | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis |