Validation Data Gallery

Filter: AllWBIHC

WB analysis using 27102-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27102-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 27102-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

LIMPII Polyclonal antibody

LIMPII Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27102-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 2 publications below

Product Information

27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25908 Product name: Recombinant human LIMPII protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-433 aa of BC021892 Sequence: NGTDGDSFHPLITKDEVLYVFPSDFCRSVYITFSDYESVQGLPAFRYKVPAEILANTSDNAGFCIPEGNCLGSGVLNVSICKNGAPIIMSFPHFYQADERFVSAIEGMHPNQEDHETFVDINPLTGIILKAAKRFQINIYVKKLDDFVETGDIRTMVFPVMYLNESVHIDKETASRLKSMINTTL 相同性解析による交差性が予測される生物種
Full Name	scavenger receptor class B, member 2
Calculated molecular weight	478 aa, 54 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	BC021892
Gene Symbol	SCARB2
Gene ID (NCBI)	950
RRID	AB_2880756
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14108
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for LIMPII antibody 27102-1-AP	Download protocol
WB protocol for LIMPII antibody 27102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Virus Res Murine models of neonatal susceptibility to a clinical strain of enterovirus A71 Authors - Jing Xie View Article
human	WB	J Virol Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage. Authors - Zhichao Pei View Article
mouse	WB,IHC	Viruses A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice. Authors - Xianliang Ke KO Validated View Article
mouse	WB, IHC	Infect Genet Evol Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body. Authors - Qingyuan Feng KD Validated View Article
mouse	WB	J Neurovirol Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. Authors - Wenjing Wang View Article
human	WB	PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness Authors - Elena Sugrue View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27102-1-AP

WB analysis of HepG2 using 27102-1-AP

WB analysis of mouse liver using 27102-1-AP

IHC staining of human liver using 27102-1-AP

IHC staining of human liver using 27102-1-AP

LIMPII Polyclonal antibody

LIMPII Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Murine models of neonatal susceptibility to a clinical strain of enterovirus A71

Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.

A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.

Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.

Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.

The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness