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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIOX Polyclonal antibody

MIOX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17623-1-AP

Synonyms

Aldehyde reductase like 6, ALDRL6, Inositol oxygenase, Kidney specific protein 32, KSP32, MI oxygenase, MIOX, myo inositol oxygenase, Renal specific oxidoreductase, RSOR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIOX Polyclonal antibody (17623-1-AP)

    WB analysis using 17623-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17623-1-AP (MIOX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using MIOX Polyclonal antibody (17623-1-AP)

    WB analysis of rat kidney using 17623-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 17623-1-AP (MIOX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MIOX Polyclonal antibody (17623-1-AP)

    IHC staining of human kidney using 17623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MIOX Polyclonal antibody (17623-1-AP)

    IHC staining of human kidney using 17623-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MIOX Polyclonal antibody (17623-1-AP)

    IHC staining of human renal cell carcinoma using 17623-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using MIOX Polyclonal antibody (17623-1-AP)

    IHC staining of human renal cell carcinoma using 17623-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17623-1-AP is MIOX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIOX fusion protein Ag11829 相同性解析による交差性が予測される生物種
Full Name myo-inositol oxygenase
Calculated molecular weight 285 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC073848
Gene symbol MIOX
Gene ID (NCBI) 55586
RRIDAB_2878421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MIOX antibody 17623-1-APDownload protocol
IHC protocol for MIOX antibody 17623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols