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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYB/c-Myb Monoclonal antibody

MYB/c-Myb Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C4A5

Cat no : 68542-1-Ig

Synonyms

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYB/c-Myb Monoclonal antibody (68542-1-Ig)

    WB analysis using 68542-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68542-1-Ig (MYB/c-Myb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using MYB/c-Myb Monoclonal antibody (68542-1-Ig)

    FC experiment of Jurkat using 68542-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MYB/c-Myb (68542-1-Ig, Clone:2C4A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68542-1-Ig targets MYB/c-Myb in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYB/c-Myb fusion protein Ag12021 相同性解析による交差性が予測される生物種
Full Name v-myb myeloblastosis viral oncogene homolog (avian)
Calculated molecular weight 640 aa, 72 kDa
Observed molecular weight40-50 kDa, 72-80 kDa
GenBank accession numberBC064955
Gene symbol c-Myb
Gene ID (NCBI) 4602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for MYB/c-Myb antibody 68542-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols