Mouse CCL11/Eotaxin ELISA Kit
Cat no : KE10043
Synonyms
Ccl11, eotaxin, Scya11
Validation Data Gallery
Product Information
KE10043 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL11 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL11. The assay recognizes mouse CCL11. An antibody specific for CCL11 has been pre-coated onto the microwells. The CCL11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL11 is added to detect the captured mouse CCL11 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CCL11/Eotaxin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.9 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20292 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 98% | 79%-109% |
| Cell culture supernatants | 111% | 103%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 226.2 | 14.3 | 6.3 |
| 2 | 20 | 57.5 | 2.7 | 4.6 |
| 3 | 20 | 13.8 | 0.6 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 242.1 | 21.0 | 8.7 |
| 2 | 24 | 57.5 | 2.8 | 5.0 |
| 3 | 24 | 15.4 | 0.5 | 3.1 |
Background Information
CCL11 also named Eotaxin, was initially discovered as an eosinophil-specific chemoattractant. CCL11 plays a central role in both allergic and non-allergic inflammatory reactions by recruiting immune cells such as eosinophils,basophils and Th2 lymphocytes. CCL11 binds to the chemokine receptors CCR2, CCR3 and CCR5, with highest affinity to CCR3. High levels of CCL11 have been described in several chronic inflammatory diseases, such as allergic rhinitis, atopic dermatitis, asthma, gastrointestinal disease and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl11, eotaxin, Scya11 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomed Pharmacother Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation. | ||
Immunol Lett Role of G-protein-coupled estrogen receptor in the pathogenesis of chronic asthma |