Mouse IL-1 alpha ELISA Kit

Sensitivity

2.7 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10024

Synonyms

IL 1, Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha



Product Information

KE10024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 alpha. The assay recognizes mouse IL-1 alpha. An antibody specific for IL-1 alpha has been pre-coated onto the microwells. The IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 alpha is added to detect the captured IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-1 alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.7 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16175

Recovery

Sample TypeAverageRange
Mouse serum87%77%-103%
Cell culture supernatants101%94%-118%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12033.92.16.1
22095.03.53.7
320388.715.03.9

InterAssay

Samplenmean ( pg/mL)SDCV%
12437.03.69.6
224117.67.36.3
324504.945.89.1

Background Information

Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms IL 1, Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, IL-1 α, Il1a, IL1alpha, IL-1alpha, IL-1α, interleukin 1 alpha, Interleukin-1 alpha, mouse IL-1 alpha

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Cell Mol Gastroenterol Hepatol

ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.

Authors - Jingwen Liu
mousecell culture supernatants

Front Immunol

Inhibitory Effects of the Lactobacillus rhamnosus GG Effector Protein HM0539 on Inflammatory Response Through the TLR4/MyD88/NF-кB Axis.

Authors - Yubin Li
mouseserum

Sci Rep

Phasic firing of dopaminergic neurons in the ventral tegmental area triggers peripheral immune responses.

Authors - Tasuku Kayama
mousecell culture supernatants

BMC Complement Med Ther

Photoprotective effects of Sargassum thunbergii on ultraviolet B-induced mouse L929 fibroblasts and zebrafish.

Authors - Bei Chen
mousecell culture supernatants

Cells

Co-Stimulation of AGEs and LPS Induces Inflammatory Mediators through PLCγ1/JNK/NF-κB Pathway in MC3T3-E1 Cells

Authors - Natsuko Tanabe
mousecell culture supernatants

Sci Total Environ

Interior decorative volatile organic compounds exposure induces sleep disorders through aberrant branched chain amino acid transaminase 2 mediated glutamatergic signaling resulting from a neuroinflammatory cascade

Authors - Jie Zheng