Mouse LIX/CXCL5 ELISA Kit
Cat no : KE10070
Synonyms
C-X-C motif chemokine 5,Cxcl5,Cytokine LIX,GCP-2(1
Validation Data Gallery
Product Information
KE10070 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse LIX/CXCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse LIX/CXCL5 . The assay recognizes mouse LIX/CXCL5. An antibody specific for mouse LIX/CXCL5 has been pre-coated onto the microwells. The mouse LIX/CXCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse LIX/CXCL5 is added to detect the captured mouse LIX/CXCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse LIX/CXCL5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 19.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 113% | 102%-124% |
| Tissue lysates | 103% | 85%-119% |
| Cell culture supernates | 106% | 82%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 930.7 | 78.7 | 8.5 |
| 2 | 20 | 223.9 | 6.9 | 3.1 |
| 3 | 20 | 47.7 | 4.8 | 10.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 787.0 | 50.9 | 6.5 |
| 2 | 24 | 180.4 | 8.4 | 4.7 |
| 3 | 24 | 31.4 | 1.8 | 5.7 |
Background Information
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). Mouse LIX can be produced by many immune cells, such as macrophages, eosinophils , as well as non-immune cells including mesothelial cells, and fibroblasts. Increased serum LIX has been observed in obesity and insulin resistance in mice and humans and is associated with complications of diabetes including nephropathy and atherosclerosis. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. According to the previous studies, cancer cell autonomous secretion of LIX as sufficient to drive infiltration of mature, protumorigenic neutrophils in a mouse model of non-small cell lung cancer (NSCLC).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-X-C motif chemokine 5,Cxcl5,Cytokine LIX,GCP-2(1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Med Nanodrug-bacteria conjugates-mediated oncogenic collagen depletion enhances immune checkpoint blockade therapy against pancreatic cancer |