Mouse/Rat Osteopontin/OPN ELISA Kit

Sensitivity

6.3 pg/mL

Range

39-2500 pg/mL

Reactivity

Mouse/Rat

Cat no : KE10097

Synonyms

AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1



Product Information

KE10097 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat OPN ELISA kit is to be used to detect and quantify protein levels of endogenous OPN. The assay recognizes mouse/rat OPN. An antibody specific for OPN has been pre-coated onto the microwells. The OPN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for OPN is added to detect the captured OPN protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse/Rat Osteopontin/OPN ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant, Urine
Assay type Sandwich
Sensitivity 6.3 pg/mL
Range 39-2500 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 20750

Recovery

Sample TypeAverageRange
Mouse serum97%85%-112%
Rat serum100%86%-130%
Cell culture supernatant106%90%-123%
Mouse urine102%91%-111%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,191.631.82.7
220275.97.92.9
32065.25.28.0

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,205.571.65.9
224283.37.82.8
32470.53.24.5

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling and is also involved in the inflammatory and immune response, oncogenesis, and cancer progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1

Publications

SpeciesSample TypeTitle
mouseserum,cell culture supernatants

Biochem Biophys Res Commun

Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts

Authors - Chao Meng