Mouse/Rat TREM-1 ELISA Kit
Sensitivity
0.4 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse/Rat
Cat no : KE10080
Synonyms
Trem1
Validation Data Gallery
Product Information
KE10080 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat TREM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat TREM-1. The assay recognizes mouse/rat TREM-1. An antibody specific for mouse/rat TREM-1 has been pre-coated onto the microwells. The mouse/rat TREM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat TREM-1 is added to detect the captured mouse/rat TREM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat TREM-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 58217 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 92% | 79%-108% |
| Mouse serum | 88% | 70%-130% |
| Rat serum | 86% | 70%-118% |
| Tissue lysates | 115% | 99%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 443.0 | 16.8 | 3.8 |
| 2 | 20 | 104.4 | 2.2 | 2.1 |
| 3 | 20 | 24.4 | 1.4 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 428.5 | 13.0 | 3.0 |
| 2 | 24 | 102.8 | 2.0 | 1.9 |
| 3 | 24 | 26.5 | 1.0 | 3.7 |
Background Information
Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. TREM1 can also exist as a soluble form (sTREM1). Increased plasma level of sTREM1 have been reported in specific diseases including Alzheimer's disease, systemic sepsis, acute myocardial infarction and subarachnoid hemorrhage.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Trem1 |