Mouse TNFSF11/RANKL ELISA Kit
Cat no : KE10035
Synonyms
Ly109l, ODF, OPG, OPGL, RANKL, Tnfsf11, Trance
Validation Data Gallery
Product Information
KE10035 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFSF11 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFSF11. The assay recognizes mouse TNFSF11. An antibody specific for TNFSF11 has been pre-coated onto the microwells. The TNFSF11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TNFSF11 is added to detect the captured TNFSF11 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Mouse TNFSF11/RANKL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21943 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 87% | 75%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 471.7 | 25.8 | 5.5 |
| 2 | 20 | 119.3 | 5.6 | 4.7 |
| 3 | 20 | 28.8 | 1.0 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 449.2 | 34.0 | 7.6 |
| 2 | 24 | 111.1 | 5.9 | 5.3 |
| 3 | 24 | 26.2 | 1.8 | 6.8 |
Background Information
TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is produced by a diverse set of lineage-specific cells and is involved in a wide variety of physiological processes that include skeletal remodeling, lymph node organogenesis, mammary gland development, and thermal regulation. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ly109l, ODF, OPG, OPGL, RANKL, Tnfsf11, Trance |
Publications
| Species | Sample Type | Title |
|---|---|---|
Mol Med Tetrandrine inhibits RANKL-induced osteoclastogenesis by promoting the degradation of TRAIL | ||
Clin Nutr Eicosapentaenoic acid supplementation modulates the osteoblast/osteoclast balance in inflammatory environments and protects against estrogen deficiency-induced bone loss in mice | ||
Polymers (Basel) Three-Dimensional Bioprinting of Strontium-Modified Controlled Assembly of Collagen Polylactic Acid Composite Scaffold for Bone Repair | ||
Immun Inflamm Dis Combined application of mesenchymal stem cells and different glucocorticoid dosing alleviates osteoporosis in SLE murine models | ||
Oral Dis Adiponectin receptors agonist alters microbiota to improve implant osseointegration in diabetic mice | ||
Adv Biol (Weinh) Regulation of Osteoblast to Osteocyte Differentiation by Cyclin-Dependent Kinase-1 |