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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUMB Polyclonal antibody

NUMB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29594-1-AP

Synonyms

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUMB Polyclonal antibody (29594-1-AP)

    WB analysis using 29594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29594-1-AP (NUMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NUMB Polyclonal antibody (29594-1-AP)

    IF Staining of A431 using 29594-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NUMB antibody (29594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29594-1-AP targets NUMB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUMB fusion protein Ag31553 相同性解析による交差性が予測される生物種
Full Name numb homolog (Drosophila)
Calculated molecular weight71KD
Observed molecular weight66-72 kDa
GenBank accession numberNM_001005743
Gene symbol NUMB
Gene ID (NCBI) 8650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUMB plays a role in the process of neurogenesis. It is required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. NUMB may also mediate local repair of brain ventricular wall damage. NUMB may play a role infurther understanding the NUMB function in development and cancer.

Protocols

Product Specific Protocols
WB protocol for NUMB antibody 29594-1-APDownload protocol
IF protocol for NUMB antibody 29594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols