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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCP2 Polyclonal antibody

PCP2 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 13774-1-AP

Synonyms

Purkinje cell protein 2, L7, GPSM4

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IHC staining of mouse eye using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IHC staining of mouse eye using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using PCP2 Polyclonal antibody (13774-1-AP)

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse cerebellum tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13774-1-AP targets PCP2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCP2 fusion protein Ag4759 相同性解析による交差性が予測される生物種
Full Name Purkinje cell protein 2
Calculated molecular weight 136 aa, 15 kDa
GenBank accession numberBC038715
Gene symbol PCP2
Gene ID (NCBI) 126006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it may function as a cell-type specific modulator for G protein-mediated cell signaling.

Protocols

Product Specific Protocols
IHC protocol for PCP2 antibody 13774-1-APDownload protocol
IF protocol for PCP2 antibody 13774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols