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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R17 Polyclonal antibody (27976-1-AP)

    WB analysis using 27976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27976-1-AP (C7orf16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP1R17 Polyclonal antibody (27976-1-AP)

    IF Staining of U2OS using 27976-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PPP1R17 Polyclonal antibody (27976-1-AP)

    IF Staining of U2OS using 27976-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PPP1R17 Polyclonal antibody

PPP1R17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27976-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

C7orf16, G substrate, GSBS, PPP1R17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27976-1-AP targets PPP1R17 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP1R17 fusion protein Ag15187 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 16
Calculated molecular weight 155 aa, 18 kDa
Observed molecular weight 20-30 kDa
GenBank accession numberBC028094
Gene Symbol PPP1R17
Gene ID (NCBI) 10842
RRIDAB_3086018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96001
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R17, an endogenous substrate for cGMP-dependent protein kinase, exists almost exclusively in cerebellar Purkinje cells, where it is possibly involved in the induction of long-term depression (PMID: 10051666). PPP1R17 inhibits phosphatase activities of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) complexes. Allelic variants increase susceptibility to hypercholesterolemia (PMID: 12955585).

Protocols

Product Specific Protocols
WB protocol for PPP1R17 antibody 27976-1-APDownload protocol
IF protocol for PPP1R17 antibody 27976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols