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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSME3 Polyclonal antibody

PSME3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30009-1-AP

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using PSME3 Polyclonal antibody (30009-1-AP)

    WB analysis of Caco-2 using 30009-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 30009-1-AP (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30009-1-AP targets PSME3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSME3 fusion protein Ag31969 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001423
Gene symbol PSME3
Gene ID (NCBI) 10197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

Protocols

Product Specific Protocols
WB protocol for PSME3 antibody 30009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols