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Nectin 3 Polyclonal antibody

Nectin 3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Nectin-3, PVRL3, PPR3, Poliovirus receptor-related protein 3, poliovirus receptor related 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11213-1-AP targets Nectin 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin 3 fusion protein Ag1712 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 3
Calculated molecular weight 61 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC017572
Gene Symbol Nectin-3
Gene ID (NCBI) 25945
RRIDAB_2269095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Nectin 3 antibody 11213-1-APDownload protocol
IHC protocol for Nectin 3 antibody 11213-1-APDownload protocol
IP protocol for Nectin 3 antibody 11213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.

Authors - Yin Zhao
humanWB

Front Microbiol

Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.

Authors - Dennis Schöttelndreier
mouse,ratWB,IF

J Neurochem

Characterization of nectin processing mediated by presenilin-dependent γ-secretase.

Authors - Kim Jinsook J
humanIHC

Biomed Pharmacother

Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.

Authors - Feixue Xu
mouseIF

Biol Reprod

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.

Authors - Przemysław Zakrzewski
humanWB,IF

Nat Chem Biol

Photoproximity labeling of endogenous receptors in the live mouse brain in minutes

Authors - Mikiko Takato
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